Regulatory

Part:BBa_J119037

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2011-12-22)


HPP START half-edge with pTet

Half-edge for use in solving a Hamiltonian Path Problem with Golden Gate Assembly. Digestion with BsmBI makes half-edge word GTGG available during simulataneous ligation. Digestion with BsaI makes START word of GTCC available.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6


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Parameters
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